Description
Recombinant full-length human HO1 was expressed in E. coli cells using an N-terminal GST tag
Scientific Background: HO1 (Heme oxygenase 1) catalyzes the oxidative cleavage of heme to biliverdin and is one of the main genes controlling heme synthesis and catabolism. HO1 plays a protective role in various disorders and can ameliorate experimental MN via multiple pathways, including anti-oxidative and immunomodulatory effects (1). Exposure of primary hepatocytes to carbon monoxide and nitric oxide results in dramatic induction of HO1 in dose- and time-dependent manner and this induction is blocked by MAP kinase inhibitors (MAPKs) but not by inhibitors of PI3 kinase pathway (2)